Attempt to clone marrow cells in spleens of lethally irradiated rhesus monkeys. SAM-TR-69-74.

An attempt was made to clone rhesus marrow cells within the spleens of lethally irradiated rhesus monkeys. Twe!e animalt received autologous transplants; 2 animals received allogeneic transplants; and 4 animals served se irradiation controls. Ten days following transplantation (or irradiation, in the case of the controls) the animals were sacrifi"d and their spleens removed. No macroscopic nodules were found in any of the spleens; microscopic examination of subserial sections revealed scatte,-d erythrocytic clones in 8 of 14 animals that received transplants. Reticulocyte co , peripheral leukocytes, and cellularity of marrow spaces indicated that the transplanted marrow had proliferated in 18 of the 14 animals with transplants, Under our conditions, significant marrow cloning did not occur in spleens of lethally irradiated rhesus monkeys in a !n.nner analogous to that. described in similar studies with rodents.